Peroxisome proliferator-activated receptor-a ligands inhibit cardiac lipoprotein lipase activity
نویسنده
چکیده
Carroll, Rogayah, and David L. Severson. Peroxisome proliferator-activated receptor-a ligands inhibit cardiac lipoprotein lipase activity. Am J Physiol Heart Circ Physiol 281: H888–H894, 2001.—Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that regulate gene expression of lipoprotein lipase (LPL) in liver and adipose tissue. We examined the direct effect of PPAR-a ligands on LPL catalytic activity in cultured cardiomyocytes from adult rat heart. After overnight culture (16 h), 1 mM Wy-14643 and 10 mM BM-17.0744 decreased total cellular LPL activity to ;50% of control with no change in enzyme synthesis or mass; as a consequence, PPAR-a activation produced a significant decrease in LPL specific activity (mU/ng LPL protein). Wy-14643 and BM-17.0744 also reduced heparin-releasable LPL activity and mass in the culture medium. Inhibition of LPL activity by Wy-14643 did not reduce the ability of insulin plus dexamethasone to stimulate cellular and heparin-releasable LPL activities. A similar inhibitory effect on cellular and heparin-releasable LPL activity was observed when cardiomyocytes were cultured with 60 mM linoleic acid. In conclusion, two different PPAR-a ligands (Wy-14643 and BM-17.0744) inhibited cellular LPL activity in cultured cardiomyocytes by a posttranscriptional and posttranslational mechanism.
منابع مشابه
Peroxisome proliferator-activated receptor-alpha ligands inhibit cardiac lipoprotein lipase activity.
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that regulate gene expression of lipoprotein lipase (LPL) in liver and adipose tissue. We examined the direct effect of PPAR-alpha ligands on LPL catalytic activity in cultured cardiomyocytes from adult rat heart. After overnight culture (16 h), 1 microM Wy-14643 and 10 microM BM-17.0744 decreased tot...
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